SANGER SEQUENCING SERVICE
IGB SANGER SEQUENCING SERVICE
1.DNA Sequencing:
DNA sequencing is a highly accurate method used for determining the nucleotide sequence of smaller DNA fragments, such as single genes, plasmids, or short amplicons in tubes or 96-well plates. This technique is essential for applications like genetic research, mutation detection, and diagnostics. Our sequencing service delivers high-quality, reliable results to support your research needs.
Workflow
Features:
- High-Quality Data Read Length: 800 to 1200 base pairs (bp)
- Turnaround Time: 3 to 5 working days
- Sequencing Analysis (can be availed for an additional charge)
- Customer Support
Service Requirements:
2.Difficult Sequencing Service:
Our Difficult Sequencing Service is designed to handle challenging DNA samples that may be more complex or less reliable for standard sequencing methods. These can include high GC content, long repetitive regions, secondary structures, or samples that are degraded or difficult to amplify. IGB uses specialized techniques and optimized protocols to deliver accurate sequencing results for these challenging cases.
Features:
- Handling Challenging Samples
- Advanced Error Reduction
- Read Length: 600 to 1000 bp, (varies based on the sample)
- Turnaround Time: 5 to 7 working days (varies based on the sample)
- Sequencing Analysis (can be availed for an additional charge)
- Customer Support
Service Requirements:
3.Fragment Analysis Service
IGB Fragment Analysis Service provides precise and accurate sizing of DNA fragments, typically used for microsatellite analysis, genotyping, and allele sizing. This service is widely applied in genetic research, forensic analysis, and biodiversity studies. Whether analyzing PCR products or small DNA fragments, IGB offer reliable results with high reproducibility.
Features:
- Precise Fragment Sizing
- Applications: Microsatellite analysis, DNA fingerprinting, allele-specific PCR, and genotyping.
- High Sensitivity
- Data Format: Electropherogram output with fragment sizes in base pairs.
- Turnaround Time: 5 to 7 working days.
- Customer Support
Sample Requirements:
4.Primer Walking Service
IGB Primer Walking Service is a powerful method for sequencing long and complex DNA regions that cannot be sequenced in a single run due to size limitations or sequence complexity. This approach uses a series of primers to “walk” along the DNA fragment, generating a contiguous and accurate sequence. It is particularly useful for sequencing large genes, genomic regions, or when dealing with difficult-to-sequence areas.
Features:
- Enables the sequencing of large DNA fragments, up to 2-10 kb or more.
- Overlapping Primers: A series of overlapping primers are designed to ensure complete sequence coverage and minimize gaps.
- High Accuracy
- Applications: Ideal for sequencing large genes, genomic regions, or complex DNA fragments.
- Turnaround Time: 7 to 14 working days, depending on the fragment size and complexity.
- Customer Support
Sample Requirements:
4.Primer Walking Service
IGB Primer Walking Service is a powerful method for sequencing long and complex DNA regions that cannot be sequenced in a single run due to size limitations or sequence complexity. This approach uses a series of primers to “walk” along the DNA fragment, generating a contiguous and accurate sequence. It is particularly useful for sequencing large genes, genomic regions, or when dealing with difficult-to-sequence areas.
5.SNP / Mutation analysis Service
IGB SNP/Mutation Analysis service ensures reliable results to help identify genetic variations that may play a key role in disease development, response to treatments, or other genetic characteristics. Whether studying known mutations or investigating novel genetic variants, IGB is here to support your research needs.
Features:
- Accurate SNP/Mutation Detection
- High Sensitivity
- Applications: Ideal for genetic research, disease diagnostics, pharmacogenomics, and personalized medicine.
- Analysis Methods: Using Sanger sequencing or high-resolution melting (HRM) analysis for precise variant detection.
- Turnaround Time: 5 to 7 working days.
- Data Interpretation: Optional sequencing analysis with interpretation available for an additional charge.
- Customer Support
Sample Requirements:
For orders and enquiry, please contact:
ImmuGenix Biosciences Private Limited,
#16/2, Nattal Garden, 1st Street,
Perambur, Chennai, – 600 011
Tamil Nadu, India.


